Human RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit
The Human RBP4 (Retinol-Binding Protein 4) Plasma ELISA Kit is designed for the accurate quantification of RBP4 levels in human plasma samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.RBP4 is a key protein involved in the transportation of retinol and is associated with metabolic disorders, such as obesity, insulin resistance, and Type 2 diabetes. It serves as a potential biomarker for assessing metabolic health and studying the mechanisms underlying these conditions.With its user-friendly protocol and reliable performance, the Human RBP4 Plasma ELISA Kit from AssayGenie provides researchers with a valuable tool for investigating the role of RBP4 in metabolic disorders and exploring potential therapeutic strategies.
Product Name:
Human RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit
SKU:
HUES02591
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
