Human Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit
The Human Regenerating Islet-Derived Protein 3 Gamma (REG3G) ELISA Kit is specifically designed for the accurate detection of REG3G levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.REG3G is a key protein involved in the regeneration and repair of tissues, particularly in the gastrointestinal tract. It plays a crucial role in maintaining gut health and has been linked to conditions such as inflammatory bowel disease and colorectal cancer.
By measuring REG3G levels, researchers can gain valuable insights into the mechanisms of tissue repair and inflammation, leading to potential therapeutic interventions.Overall, the REG3G ELISA Kit is an essential tool for studying the role of REG3G in various physiological and pathological processes, providing valuable data for advancing our understanding of tissue regeneration and disease development.
Product Name:
Human Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit
Product Code:
HUDL02520
Size:
96 Assays
Target:
REG3g
Synonyms:
REG-III, PAP1B, PAPIB, UNQ429, Pancreatitis-associated protein 1B, Regenerating islet-derived protein III-gamma
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
