The Human Renin ELISA Kit from AssayGenie is a cutting-edge tool designed for the precise measurement of renin levels in human samples. Renin is a key enzyme in the Renin-Angiotensin System (RAS), which plays a critical role in regulating blood pressure and fluid balance in the body. This ELISA kit offers superior sensitivity and specificity, ensuring accurate and reproducible results in serum, plasma, and cell culture supernatants. Its easy-to-use format makes it suitable for a wide range of research applications, including studies on hypertension, kidney diseases, and cardiovascular disorders. By accurately quantifying renin levels, researchers can gain valuable insights into the pathophysiology of various diseases and potentially identify new therapeutic targets. Trust the Human Renin ELISA Kit from AssayGenie for reliable and impactful research outcomes.
Product Name:
Human REN (Renin) ELISA Kit
SKU:
HUES01403
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
