Description
Human REST Recombinant Protein (N-His)
Human REST Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Glu748-Glu1097, with an N-terminal His tag. Predicted molecular weight: 40.61 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RE1-silencing transcription factor (REST) is a ~121 kDa protein. Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. For research use only.
Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 3.
| Product Name: | Human REST Recombinant Protein (N-His) |
| SKU: | GXRP02158 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RE1-silencing transcription factor, Neural-restrictive silencer factor, NRSF, X2 box repressor, XBR, REST |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Glu748-Glu1097 |
| Calculated MW: | 40.61 kDa |
| UniProt: | Q13127 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
