Human Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit
The Human Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit is specifically designed for the quantitative detection of ARHGEF7 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it an ideal tool for various research applications.ARHGEF7, also known as beta-PIX, is a key regulator of Rho GTPase signaling pathways involved in cell migration, adhesion, and cytoskeletal dynamics. Dysregulation of ARHGEF7 has been implicated in various pathological conditions, including cancer, cardiovascular diseases, and neurological disorders, highlighting its significance as a potential therapeutic target and biomarker.
By utilizing the Human ARHGEF7 ELISA Kit, researchers can gain valuable insights into the role of ARHGEF7 in disease progression and develop novel strategies for targeted therapy. With its ease of use and accurate results, this kit is essential for advancing our understanding of ARHGEF7 biology and its implications in human health.
Product Name:
Human Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
