Human RNA Binding Motif Protein 3 (RBM3) ELISA Kit
The Human RNA-Binding Motif Protein 3 (RBM3) ELISA Kit is a cutting-edge tool designed for the precise measurement of RBM3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and dependable results that are essential for a wide range of research applications.RBM3 is a vital protein that plays a crucial role in RNA metabolism and is known to be involved in various biological processes such as cell survival, stress response, and neuroprotection.
Dysregulation of RBM3 has been linked to several diseases including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Invest in the Human RNA-Binding Motif Protein 3 (RBM3) ELISA Kit today and empower your research with reliable data to advance the understanding of RBM3 biology and its implications in human health and disease.
Product Name:
Human RNA Binding Motif Protein 3 (RBM3) ELISA Kit
Product Code:
HUDL02511
Size:
96 Assays
Target:
RBM3
Synonyms:
IS1-RNPL, RNPL, Putative RNA-binding protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
