Description
Human RNF168 Recombinant Protein (N-His)
Human RNF168 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Gln95, with an N-terminal His tag. Predicted molecular weight: 13.08 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase RNF168 is a ~65 kDa protein. E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. For research use only.
Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair.
| Product Name: | Human RNF168 Recombinant Protein (N-His) |
| SKU: | GXRP07631 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | E3 ubiquitin-protein ligase RNF168, hRNF168, 2.3.2.27, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Gln95 |
| Calculated MW: | 13.08 kDa |
| UniProt: | Q8IYW5 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
