Description
Human RNF20 Recombinant Protein (N-GST & C-His)
Human RNF20 Recombinant Protein (N-GST & C-His) is a recombinant protein expressed in E. coli, spanning Asp906-Gly975, with an N-terminal GST tag and a C-terminal His tag. Predicted molecular weight: 36.47 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase BRE1A is a ~113 kDa protein. Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). For research use only.
H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
| Product Name: | Human RNF20 Recombinant Protein (N-GST & C-His) |
| SKU: | GXRP12062 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | BRE1-A, BRE1A, E3 ubiquitin-protein ligase BRE1A, EC:2.3.2.27, RING finger protein 20, RING-type E3 ubiquitin transferase BRE1A, RNF20, hBRE1 |
| Tag: | N-GST & C-His |
| Expression Host: | E. coli |
| Protein Length: | Asp906-Gly975 |
| Calculated MW: | 36.47 kDa |
| UniProt: | Q5VTR2 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
