Description
Human RNF4 Recombinant Protein (N-GST & C-His)
Human RNF4 Recombinant Protein (N-GST & C-His) is a recombinant protein expressed in E. coli, spanning Thr129-Ile190, with an N-terminal GST tag and a C-terminal His tag. Predicted molecular weight: 35.23 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase RNF4 is a ~21 kDa protein. E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. For research use only.
Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.
| Product Name: | Human RNF4 Recombinant Protein (N-GST & C-His) |
| SKU: | GXRP09431 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | E3 ubiquitin-protein ligase RNF4; 2.3.2.27; RING finger protein 4; Small nuclear ring finger protein; Protein SNURF; RNF4; SNURF; RES4-26 |
| Tag: | N-GST & C-His |
| Expression Host: | E. coli |
| Protein Length: | Thr129-Ile190 |
| Calculated MW: | 35.23 kDa |
| UniProt: | P78317 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
