Description
Human RNGTT Recombinant Protein (N-His)
Human RNGTT Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Pro328-Val553, with an N-terminal His tag. Predicted molecular weight: 28.41 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. mRNA-capping enzyme (RNGTT) is a ~68 kDa protein. Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. For research use only.
Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate.
| Product Name: | Human RNGTT Recombinant Protein (N-His) |
| SKU: | GXRP07403 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | mRNA-capping enzyme, HCAP1, HCE, mRNA 5'-triphosphate monophosphatase, 3.6.1.74, mRNA 5'-phosphatase, mRNA guanylyltransferase, 2.7.7.50, GTP--RNA guanylyltransferase, GTase, RNGTT, CAP1A |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Pro328-Val553 |
| Calculated MW: | 28.41 kDa |
| UniProt: | O60942 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |