Description
Human RPS6KA3 Recombinant Protein (N-His)
Human RPS6KA3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp64-Arg383, with an N-terminal His tag. Predicted molecular weight: 39.07 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ribosomal protein S6 kinase alpha-3 (RPS6KA3) is a ~83 kDa protein. Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. For research use only.
In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex.
| Product Name: | Human RPS6KA3 Recombinant Protein (N-His) |
| SKU: | GXRP05069 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Insulin-stimulated protein kinase 1, MAP kinase-activated protein kinase 1b, ISPK-1, RSK2, Ribosomal S6 kinase 2, MAPKAPK-1b, ISPK1, pp90RSK2, p90RSK3, RSK-2, 90 kDa ribosomal protein S6 kinase 3, MAPKAPK1B, S6K-alpha-3, MAPKAP kinase 1b, p90-RSK 3, RPS6KA3, MAPK-activated protein kinase 1b, Ribosomal protein S6 kinase alpha-3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp64-Arg383 |
| Calculated MW: | 39.07 kDa |
| UniProt: | P51812 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
