Human S100 Calcium Binding Protein A14 (S100A14) ELISA Kit
The Human S100 Calcium Binding Protein A14 (S100A14) ELISA Kit is specifically designed for the precise detection of S100A14 levels in human serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, ensuring accurate and consistent results for various research applications.S100A14 is a calcium-binding protein that plays a critical role in cell signaling and regulation. It is associated with various diseases, including cancer and inflammatory conditions, making it a valuable biomarker for studying these diseases and identifying potential treatment options.
By using the Human S100A14 ELISA Kit, researchers can confidently measure S100A14 levels in biological samples, providing important insights into disease mechanisms and potential therapeutic targets. Trust in this kit for high-quality results in your research endeavors.
Product Name:
Human S100 Calcium Binding Protein A14 (S100A14) ELISA Kit
Product Code:
HUDL02583
Size:
96 Assays
Target:
S100A14
Synonyms:
S100-A14, S100-A15, S100A15, BCMP84, S114
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
