Description
Human SAR1B Recombinant Protein (N-His)
Human SAR1B Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Asp198, with an N-terminal His tag. Predicted molecular weight: 24.57 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Small COPII coat GTPase SAR1B is a ~22 kDa protein. Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. For research use only.
The active GTP-bound form inserts into the endoplasmic reticulum membrane where it recruits the remainder of the coat protein complex II/COPII. The coat protein complex II assembling and polymerizing on endoplasmic reticulum membrane is responsible for both the sorting of cargos and the deformation and budding of membranes into vesicles destined to the Golgi. In contrast to SAR1A, SAR1B specifically interacts with the cargo receptor SURF4 to mediate the transport of lipid-carrying lipoproteins including APOB and APOA1 from the endoplasmic reticulum to the Golgi and thereby, indirectly regulates lipid homeostasis. In addition to its role in vesicle trafficking, can also function as a leucine sensor regulating TORC1 signaling and more indirectly cellular metabolism, growth and survival.
| Product Name: | Human SAR1B Recombinant Protein (N-His) |
| SKU: | GXRP06440 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | GTP-binding protein SAR1b, SAR1B, SARB, GTP-binding protein B, SARA2, GTBPB |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Asp198 |
| Calculated MW: | 24.57 kDa |
| UniProt: | Q9Y6B6 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
