Human sCD163 (Soluable Cluster of Differentiation 163) ELISA Kit
The Human SCD163 (Soluable Cluster of Differentiation 163) ELISA Kit is specially designed for the accurate measurement of SCD163 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and reliable results for various research applications.SCD163, also known as CD163, is a key protein involved in the regulation of inflammation and immune responses. It plays a crucial role in conditions such as sepsis, liver disease, and cardiovascular disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions. With its high-performance features, the Human SCD163 ELISA Kit is an essential tool for researchers looking to explore the role of SCD163 in health and disease, offering precise and accurate measurement of this important protein level in human samples.
Product Name:
Human sCD163 (Soluable Cluster of Differentiation 163) ELISA Kit
SKU:
HUES01323
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
