The Human SEMA3A (Semaphorin 3A) CLIA Kit is a powerful tool for accurately measuring levels of SEMA3A in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.SEMA3A is a key signaling protein involved in neuronal development, immune regulation, and cancer progression.
By studying SEMA3A levels, researchers can gain valuable insights into the mechanisms underlying these processes and potentially identify new therapeutic targets for related diseases.Overall, the Human SEMA3A CLIA Kit is an indispensable resource for researchers seeking to further explore the role of SEMA3A in various physiological and pathological contexts.
Product Name:
Human SEMA3A (Semaphorin 3A) CLIA Kit
SKU:
HUES00730
Target:
Human SEMA3A (Semaphorin 3A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA3A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA3A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SEMA3A. You can calculate the concentration of Human SEMA3A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
89-102
88-100
Average (%)
101
94
93
1:4
Range (%)
95-111
98-113
94-109
Average (%)
102
103
100
1:8
Range (%)
93-107
89-99
92-105
Average (%)
99
94
99
1:16
Range (%)
92-108
102-117
94-110
Average (%)
100
110
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.04
315.47
856.96
107.94
314.58
772.72
Standard deviation
10.87
23.44
81.67
12.73
34.35
55.33
C V (%)
10.87
7.43
9.53
11.79
10.92
7.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SEMA3A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SEMA3A in samples. No significant cross-reactivity or interference between Human SEMA3A and analogues was observed.
