The Human SEMA3A (Semaphorin 3A) ELISA Kit is a powerful tool for the quantitative measurement of SEMA3A levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reliable results, making it perfect for a variety of research applications.SEMA3A is a critical protein involved in axon guidance and neural development, playing a key role in neuronal growth and cell signaling. Dysregulation of SEMA3A has been implicated in various neurological disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human SEMA3A ELISA Kit offers a comprehensive solution for researchers looking to investigate the role of SEMA3A in health and disease, providing valuable insights into neural development and potential therapeutic targets.
Product Name:
Human SEMA3A (Semaphorin 3A) ELISA Kit
SKU:
HUES02332
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
