Human Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) ELISA Kit
The Human Serine Palmitoyltransferase Long Chain Base Subunit 1 (SPTLC1) ELISA Kit is a cutting-edge assay designed for the accurate measurement of SPTLC1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SPTLC1 is a vital enzyme involved in the biosynthesis of sphingolipids, which are essential for maintaining cell structure and function. Dysregulation of sphingolipid metabolism has been linked to a range of human diseases, including neurodegenerative disorders, metabolic disorders, and cancer.
The Human SPTLC1 ELISA Kit provides researchers with a valuable tool for studying the role of SPTLC1 in these conditions and exploring potential therapeutic interventions.Overall, the Human SPTLC1 ELISA Kit offers researchers a reliable and efficient method for quantifying SPTLC1 levels in biological samples, facilitating in-depth investigations into the role of sphingolipid metabolism in health and disease.
Product Name:
Human Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) ELISA Kit
Product Code:
HUDL02766
Size:
96 Assays
Target:
SPTLC1
Synonyms:
HSAN, HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI, Hereditary Sensory Neuropathy,Type 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1
Detection Method:
Sandwich
Range:
78.12-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
Torretta et al.
Severity of COVID-19 Patients Predicted by Serum Sphingolipids Signature
