Human Serpin A6/Corticosteroid-binding globulin ELISA Kit
The Human Serpin A6 (Corticosteroid-binding globulin) ELISA Kit is a comprehensive tool designed for the accurate quantification of serpin A6 levels in human serum, plasma, and cell culture supernatants. Utilizing advanced technology, this kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications. Serpin A6, also known as corticosteroid-binding globulin, is a vital protein involved in regulating the concentration of cortisol in the blood. Dysregulation of serpin A6 levels has been linked to various health conditions, including metabolic disorders, inflammatory diseases, and stress-related ailments.
Therefore, measuring serpin A6 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted treatments. With its user-friendly protocol and innovative features, the Human Serpin A6 ELISA Kit is a valuable asset for researchers seeking to explore the role of serpin A6 in health and disease. Its reliable performance and accurate results make it an indispensable tool for advancing scientific knowledge and improving patient outcomes.
Product Name:
Human Serpin A6/Corticosteroid-binding globulin ELISA Kit
SKU:
HUFI00544
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 ng/mL
Range:
15.625-1000 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-104%
91-101%
85-105%
EDTA Plasma (n = 5)
84-99%
85-91%
88-100%
Heparin Plasma (n = 5)
82-96%
90-98%
82-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-104
92
EDTA Plasma (n = 5)
92-101
96
Heparin Plasma (n = 5)
87-105
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
