Description
Human SET Recombinant Protein (N-His)
Human SET Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys90-Asn228, with an N-terminal His tag. Predicted molecular weight: 18.56 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein SET is a ~33 kDa protein. Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. For research use only.
Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases.
| Product Name: | Human SET Recombinant Protein (N-His) |
| SKU: | GXRP04962 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Protein SET, Phosphatase 2A inhibitor I2PP2A, SET, HLA-DR-associated protein II, PHAPII, TAF-I, I-2PP2A, Inhibitor of granzyme A-activated DNase, IGAAD, Template-activating factor I |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys90-Asn228 |
| Calculated MW: | 18.56 kDa |
| UniProt: | Q01105 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
