Description
Human SHH Recombinant Protein (N-His)
Human SHH Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Pro36-Ala193, with an N-terminal His tag. Predicted molecular weight: 20.21 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Sonic hedgehog protein (SHH) is a ~49 kDa protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. For research use only.
Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the endoplasmic reticulum. Once cleaved, ShhC is degraded in the endoplasmic reticulum.
| Product Name: | Human SHH Recombinant Protein (N-His) |
| SKU: | GXRP02322 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ShhNC, Sonic hedgehog protein, Shh N-terminal processed signaling domains, SHH, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhN, ShhNp, HHG-1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Pro36-Ala193 |
| Calculated MW: | 20.21 kDa |
| UniProt: | Q15465 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
