Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3) ELISA Kit
The Human SPLUNC3 (Short Palate, Lung, and Nasal Epithelium Carcinoma-Associated Protein 3) ELISA Kit is a reliable tool for accurately measuring SPLUNC3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring conclusive and reproducible results for various research purposes.SPLUNC3 is a protein that plays a vital role in regulating lung health and immune responses. Dysregulation of SPLUNC3 has been associated with respiratory diseases and lung cancer, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
This ELISA Kit provides researchers with a valuable tool for investigating the role of SPLUNC3 in disease pathogenesis and developing targeted treatment strategies. Its accuracy and precision make it suitable for a wide range of research applications in the fields of pulmonology, oncology, and immunology.
Product Name:
Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3) ELISA Kit
Product Code:
HUDL02760
Size:
96 Assays
Target:
SPLUNC3
Synonyms:
BPIFA3, C20orf71, BPI fold-containing family A member 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
