The Human Sjogren Syndrome Antigen B (SSB) ELISA Kit is a cutting-edge diagnostic tool designed for the precise detection of SSB antigen levels in human serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The SSB antigen is a key biomarker for Sjogren's syndrome, an autoimmune disorder that primarily affects the salivary glands and tear ducts. By detecting SSB antigen levels, researchers can gain valuable insights into the pathogenesis of Sjogren's syndrome and develop innovative diagnostic and therapeutic strategies.
The Human Sjogren Syndrome Antigen B (SSB) ELISA Kit is a essential tool for researchers and clinicians alike, providing a comprehensive solution for studying and diagnosing this complex autoimmune disease. Trust in the accuracy and reliability of this kit to advance your research and improve patient outcomes.
Product Name:
Human Sjogren Syndrome Antigen B (SSB) ELISA Kit
Product Code:
HUDL02775
Size:
96 Assays
Target:
SSB
Synonyms:
LARP3, Lupus La Protein, Autoantigen La, La Ribonucleoprotein Domain Family,Member 3, Autoantigen SSB/La
Detection Method:
Sandwich
Range:
78.12-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
