Description
Human SMO Recombinant Protein (N-His)
Human SMO Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser32-Tyr233, with an N-terminal His tag. Predicted molecular weight: 24.21 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein smoothened is a ~86 kDa protein. G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. For research use only.
Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes. Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.
| Product Name: | Human SMO Recombinant Protein (N-His) |
| SKU: | GXRP02785 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | SMOH, Smoothened homolog, SMO, Protein Gx |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser32-Tyr233 |
| Calculated MW: | 24.21 kDa |
| UniProt: | Q99835 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
