Description
Human SMURF2 Recombinant Protein (N-His)
Human SMURF2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning His246-Glu400, with an N-terminal His tag. Predicted molecular weight: 20.32 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase SMURF2 is a ~86 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. For research use only.
Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1. Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation. SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation.
| Product Name: | Human SMURF2 Recombinant Protein (N-His) |
| SKU: | GXRP02876 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | SMAD ubiquitination regulatory factor 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, SMURF2, SMAD-specific E3 ubiquitin-protein ligase 2, E3 ubiquitin-protein ligase SMURF2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | His246-Glu400 |
| Calculated MW: | 20.32 kDa |
| UniProt: | Q9HAU4 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
