SynonymsSNAPIN,SNAP-25-Binding Protein,Biogenesis of lysosome-related organelles complex 1 subunit 7,Synaptosomal-associated protein 25-binding protein
ReactivityHuman
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
HUDL02729
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O95295
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SNAPIN,SNAP-25-Binding Protein,Biogenesis of lysosome-related organelles complex 1 subunit 7,Synaptosomal-associated protein 25-binding protein
The Human SNAP-associated protein (SNAPAP) ELISA Kit from AssayGenie is a reliable and accurate tool for detecting SNAPAP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.SNAPAP is a protein that is associated with SNAP receptors, playing a crucial role in intracellular vesicle trafficking and membrane fusion processes. Dysregulation of SNAPAP has been linked to various diseases, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.
With the AssayGenie SNAPAP ELISA Kit, researchers can confidently measure SNAPAP levels in human samples, gaining insights into its role in disease pathology and potential avenues for targeted treatment strategies. Elevate your research with the precision and reliability of the AssayGenie SNAPAP ELISA Kit.
Product Name:
Human SNAP Associated Protein (SNAPAP) ELISA Kit
Product Code:
HUDL02729
Size:
96 Assays
Target:
SNAPAP
Synonyms:
SNAPIN, SNAP-25-Binding Protein, Biogenesis of lysosome-related organelles complex 1 subunit 7, Synaptosomal-associated protein 25-binding protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
