Description
Human SPARC Recombinant Protein (N-His)
Human SPARC Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala18-Ile303, with an N-terminal His tag. Predicted molecular weight: 34.99 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. SPARC (Osteonectin) is a ~34 kDa protein. Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. For research use only.
Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
| Product Name: | Human SPARC Recombinant Protein (N-His) |
| SKU: | GXRP00778 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Secreted protein acidic and rich in cysteine, Basement-membrane protein 40, BM-40, Osteonectin, ON, SPARC |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala18-Ile303 |
| Calculated MW: | 34.99 kDa |
| UniProt: | P09486 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
