Description
Human SPOUT1 Recombinant Protein (N-His)
Human SPOUT1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr74-Thr376, with an N-terminal His tag. Predicted molecular weight: 35.35 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. 28S rRNA (uridine-N(3))-methyltransferase is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. For research use only.
Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis.
| Product Name: | Human SPOUT1 Recombinant Protein (N-His) |
| SKU: | GXRP11575 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | 28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr74-Thr376 |
| Calculated MW: | 35.35 kDa |
| UniProt: | Q5T280 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
