Description
Human SPTLC1 Recombinant Protein (N-His)
Human SPTLC1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser90-Lys437, with an N-terminal His tag. Predicted molecular weight: 40.86 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Serine palmitoyltransferase 1 is a ~52 kDa protein. Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. For research use only.
The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA.
| Product Name: | Human SPTLC1 Recombinant Protein (N-His) |
| SKU: | GXRP10421 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:2.3.1.50, LCB 1, LCB1, Long chain base biosynthesis protein 1, SPT 1, SPT1, SPTLC1, Serine palmitoyltransferase 1, Serine-palmitoyl-CoA transferase 1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser90-Lys437 |
| Calculated MW: | 40.86 kDa |
| UniProt: | O15269 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
