The Human SPX (Spexin) ELISA Kit is specifically designed for the quantification of SPX levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing accurate and consistent results for various research applications.SPX, also known as spexin, is a novel peptide hormone that regulates various physiological processes including metabolism, appetite, and stress response. It has been implicated in conditions such as obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and developing potential treatments.With its easy-to-use format and reliable performance, the Human SPX ELISA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of SPX in human health and disease.
Product Name:
Human SPX (Spexin) ELISA Kit
SKU:
HUES03578
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
