Description
Human SRSF1 Recombinant Protein (N-His)
Human SRSF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Cys16-Arg224, with an N-terminal His tag. Predicted molecular weight: 25.8 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Serine/arginine-rich splicing factor 1 (SRSF1) is a ~27 kDa protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. For research use only.
Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro.
| Product Name: | Human SRSF1 Recombinant Protein (N-His) |
| SKU: | GXRP02096 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Splicing factor, arginine/serine-rich 1, ASF, ASF-1, Serine/arginine-rich splicing factor 1, SF2P33, SFRS1, SRSF1, SF2, Alternative-splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Cys16-Arg224 |
| Calculated MW: | 25.8 kDa |
| UniProt: | Q07955 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
