Human sTfR1 (Soluble Transferrin Receptor 1) ELISA Kit
The Human sTfR1 (Soluble Transferrin Receptor 1) ELISA Kit is meticulously crafted to enable the precise quantitative assessment of Soluble Transferrin Receptor 1 levels in diverse human biological samples. Soluble Transferrin Receptor 1 plays a crucial role in the regulation of iron homeostasis by reflecting the cellular iron demand and transport. Accurate measurement of sTfR1 is essential for understanding iron metabolism, hematological disorders, and various iron-related pathologies. Our ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in detecting sTfR1 levels. Developed under stringent quality control protocols, this kit exhibits robust performance and ease of use, making it a top choice for both research and clinical applications. Rely on Assay Genie's Human sTfR1 ELISA Kit for dependable quantification of this vital biomarker in your investigative studies.
Product Name:
Human sTfR1 (Soluble Transferrin Receptor 1) ELISA Kit
SKU:
AEES00121
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
