Human Surfactant Associated Protein B (SPB) ELISA Kit
The Human Surfactant Associated Protein B (SP-B) ELISA Kit is specifically designed for the accurate and precise detection of SP-B levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers in various fields.Surfactant Associated Protein B is a critical component of lung surfactant, crucial for maintaining alveolar stability and preventing collapse. Deficiencies in SP-B can lead to respiratory distress syndrome in newborns and other respiratory conditions in adults.
Therefore, measuring SP-B levels can provide valuable insights into lung health and disease progression.This ELISA kit is an essential tool for studying respiratory diseases, pulmonary function, and lung injury, as well as for monitoring treatment responses and developing potential therapies. With its easy-to-use protocol and high-performance characteristics, the Human Surfactant Associated Protein B ELISA Kit is an indispensable resource for researchers investigating lung biology and pulmonary disorders.
Product Name:
Human Surfactant Associated Protein B (SPB) ELISA Kit
Product Code:
HUDL02751
Size:
96 Assays
Target:
SPB
Synonyms:
SFTPB, PSPB, SFTB3, SFTP3, SP-B, Pulmonary Surfactant Protein B, 18 kDa pulmonary-surfactant protein, 6 kDa protein
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
