Human TAT (Thrombin/Antithrombin Complex) CLIA Kit
The Human TAT (Thrombin-Antithrombin Complex) CLIA Kit is a vital tool for the accurate measurement of TAT levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications. The Thrombin-Antithrombin Complex is a critical marker of blood coagulation and thrombotic activity, making it indispensable for studying conditions such as thrombosis, embolism, and other related disorders.
Understanding TAT levels can provide valuable insights into the mechanisms of coagulation and help guide the development of effective treatments.With its advanced technology and reliable performance, the Human TAT CLIA Kit is an essential tool for researchers and healthcare professionals seeking to investigate and monitor thrombotic activities in both clinical and research settings.
Product Name:
Human TAT (Thrombin/Antithrombin Complex) CLIA Kit
SKU:
HUES00994
Target:
Human TAT (Thrombin/Antithrombin Complex)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TAT. You can calculate the concentration of Human TAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
98-113
90-103
Average (%)
95
104
96
1:4
Range (%)
88-102
101-116
99-113
Average (%)
94
106
105
1:8
Range (%)
85-95
102-117
84-97
Average (%)
90
108
90
1:16
Range (%)
93-106
98-113
89-103
Average (%)
98
105
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
106
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
97-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.4
51.93
228.77
22.75
50.64
207.6
Standard deviation
2.41
5.36
18.46
2.2
5.96
22.21
C V (%)
10.3
10.32
8.07
9.67
11.77
10.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TAT in samples. No significant cross-reactivity or interference between Human TAT and analogues was observed.
