Human TERT (Telomerase Reverse Transcriptase) ELISA Kit
The Human TERT (Telomerase Reverse Transcriptase) ELISA Kit is specifically designed for the precise detection of TERT levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results, perfect for various research purposes.TERT is a key enzyme involved in maintaining telomere length, crucial for cell division and genomic stability. Its dysregulation has been linked to aging, cancer, and other diseases, highlighting its importance as a biomarker for studying these conditions and exploring potential treatments.Overall, the Human TERT ELISA Kit from Assay Genie is an invaluable tool for researchers looking to uncover the role of TERT in health and disease, providing accurate and reliable data for in-depth analyses.
Product Name:
Human TERT (Telomerase Reverse Transcriptase) ELISA Kit
SKU:
HUES01857
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
