The Human Testis Anion Transporter 1 (TAT1) ELISA Kit is a cutting-edge tool designed for the precise measurement of TAT1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering accurate and consistent results, making it an excellent choice for various research purposes.TAT1 is a critical transporter protein involved in the regulation of anion transport in the testis. Its dysfunction or dysregulation has been linked to various reproductive disorders and infertility, making it a significant target for research in the field of reproductive biology.
By accurately measuring TAT1 levels, researchers can gain valuable insights into its role in male fertility and reproductive health.In conclusion, the Human Testis Anion Transporter 1 (TAT1) ELISA Kit is a valuable tool for researchers looking to explore the role of TAT1 in reproductive biology and further understand its implications in male fertility and reproductive disorders. With its high sensitivity and specificity, this kit provides reliable results, aiding in the advancement of scientific knowledge in this field.
Product Name:
Human Testis Anion Transporter 1 (TAT1) ELISA Kit
Product Code:
HUDL02833
Size:
96 Assays
Target:
TAT1
Synonyms:
SLC26A8, Solute Carrier Family 26 Member 8, Anion exchange transporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
