The Human Thymosin beta-10 (TMSB10) ELISA Kit is designed for the precise measurement of TMSB10 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.Thymosin beta-10 is a key protein involved in cell migration, wound healing, and tissue regeneration. Its dysregulation has been linked to various diseases, including cancer, fibrosis, and inflammatory disorders.
By detecting and quantifying TMSB10 levels, researchers can gain valuable insights into the role of this protein in disease progression and potentially identify new therapeutic targets.Overall, the Human Thymosin beta-10 (TMSB10) ELISA Kit is a valuable tool for studying the biological functions of TMSB10 and its implications in various pathological conditions. Its high performance and accuracy make it an essential asset for researchers in the field of cell biology, immunology, and oncology.
Product Name:
Human Thymosin Beta 10 (TMSb10) ELISA Kit
Product Code:
HUDL02928
Size:
96 Assays
Target:
TMSb10
Synonyms:
MIG12, TB10, PTMB10
Detection Method:
Competition
Range:
7.812-500ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
