The Human Tight Junction Protein 2 (TJP2) ELISA Kit is a powerful tool for detecting and quantifying levels of TJP2 in human biological samples. Tight junction proteins play a critical role in maintaining the integrity of epithelial and endothelial barriers, regulating the passage of ions, molecules, and cells across cell layers.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reliable results for researchers studying tight junction function and dysfunction in various disease states.
TJP2 is involved in the assembly and regulation of tight junction complexes, making it a valuable target for understanding conditions such as inflammatory bowel disease, cancer metastasis, and barrier dysfunction in neurological disorders.By utilizing the Human TJP2 ELISA Kit, researchers can gain valuable insights into the role of tight junction proteins in disease pathogenesis and explore potential therapeutic strategies for targeting TJP2 in the treatment of various disorders.
Product Name:
Human Tight Junction Protein 2 (TJP2) ELISA Kit
Product Code:
HUDL02905
Size:
96 Assays
Synonyms:
X104, ZO-2, ZO2, Zona Occludens 2, Friedreich Ataxia Region Gene X104, Zona occludens protein 2, Zonula occludens protein 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
