Description
Human TOP3A Recombinant Protein (N-His)
Human TOP3A Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys35-Ser179, with an N-terminal His tag. Predicted molecular weight: 19.17 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA topoisomerase 3-alpha (TOP3A) is a ~112 kDa protein. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. For research use only.
Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
| Product Name: | Human TOP3A Recombinant Protein (N-His) |
| SKU: | GXRP02202 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DNA topoisomerase 3-alpha, TOP3, TOP3A, DNA topoisomerase III alpha |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys35-Ser179 |
| Calculated MW: | 19.17 kDa |
| UniProt: | Q13472 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
