Human tPA (Plasminogen Activator, Tissue) CLIA Kit
The Human TPA (Plasminogen Activator) ELISA Kit is specifically designed for the precise measurement of TPA levels in human tissue samples, offering a high level of sensitivity and specificity for accurate and reliable results. TPA is a key enzyme that plays a vital role in the breakdown of blood clots, making it a crucial biomarker for cardiovascular diseases, thrombosis, and other related conditions. By accurately measuring TPA levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially develop novel therapeutic strategies.
This CLIA kit is suitable for use with human serum, plasma, and cell culture supernatants, providing researchers with a versatile tool for studying the physiological and pathological mechanisms involving TPA. Trust in the Human TPA ELISA Kit for dependable results and meaningful discoveries in your research endeavors.
Product Name:
Human tPA (Plasminogen Activator, Tissue) CLIA Kit
SKU:
HUES01124
Target:
Human tPA (Plasminogen Activator, Tissue)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human tPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human tPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human tPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human tPA. You can calculate the concentration of Human tPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
88-100
88-101
Average (%)
94
94
93
1:4
Range (%)
101-115
91-106
86-98
Average (%)
108
98
93
1:8
Range (%)
91-106
95-111
94-106
Average (%)
98
103
100
1:16
Range (%)
91-104
101-118
99-115
Average (%)
96
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
104
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
94-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
368.54
1241.54
3545.67
345.98
1320.62
3302.68
Standard deviation
36.96
123.66
281.53
41.93
123.61
352.07
C V (%)
10.03
9.96
7.94
12.12
9.36
10.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human tPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human tPA in samples. No significant cross-reactivity or interference between Human tPA and analogues was observed.
