Human tPA (Plasminogen Activator, Tissue) ELISA Kit
The Human Tissue Plasminogen Activator (tPA) ELISA Kit is a reliable and accurate tool for the quantitative detection of tPA levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.tPA, also known as tissue-type plasminogen activator, is a critical enzyme involved in the breakdown of blood clots and regulation of fibrinolysis. Dysregulation of tPA has been associated with various cardiovascular diseases, stroke, and other thrombotic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. This ELISA kit is designed to streamline the detection of tPA levels in human samples, providing researchers with a powerful tool for investigating the role of tPA in disease pathogenesis and therapeutic development. Get accurate and reliable results with the Human tPA ELISA Kit from Assay Genie.
Product Name:
Human tPA (Plasminogen Activator, Tissue) ELISA Kit
SKU:
HUES03015
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
