Human Transcobalamin II, Macrocytic Anemia (TCN2) ELISA Kit
The Human Transcobalamin II ELISA Kit is specifically designed for the precise measurement of Transcobalamin II levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results, making it an ideal tool for various research applications.Transcobalamin II is a vital protein that plays a crucial role in the transport and delivery of vitamin B12 to cells in the body. Deficiencies in Transcobalamin II have been linked to macrocytic anemia, a condition characterized by oversized red blood cells, leading to symptoms such as fatigue and weakness.
By accurately measuring Transcobalamin II levels, researchers can better understand the mechanisms behind macrocytic anemia and potentially develop targeted treatments for this condition. The Human Transcobalamin II ELISA Kit provides a reliable method for studying Transcobalamin II levels and their implications in various health conditions.
Product Name:
Human Transcobalamin II, Macrocytic Anemia (TCN2) ELISA Kit
Product Code:
HUDL02846
Size:
96 Assays
Target:
TCN2
Synonyms:
TC2, Transcobalamin II
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
