Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit
The Human Transcription Factor Binding to IGHM Enhancer 3 (TFE3) ELISA Kit is specifically designed for the precise measurement of TFE3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research studies.TFE3 is a transcription factor that plays a critical role in gene regulation and cellular processes, making it a key player in various biological pathways. Its interaction with the IGHM enhancer 3 region has important implications in gene expression and cellular function, making it a valuable target for studying disease mechanisms and potential therapeutic interventions.
Whether investigating cancer biology, immunology, or developmental biology, the Human TFE3 ELISA Kit provides researchers with a powerful tool to explore the intricate molecular mechanisms underlying these processes. With its high-quality components and user-friendly protocol, this kit is a versatile and essential resource for any laboratory studying transcription factor biology.
Product Name:
Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit
Product Code:
HUDL02858
Size:
96 Assays
Synonyms:
RCCP2, TFEA, BHLHE33, Transcription Tactor E Family Member A, Class E basic helix-loop-helix protein 33, Transcription factor E3
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
