Human Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit
The Human Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit is specifically designed for the quantitative measurement of TGFb2 levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and reproducible results, making it ideal for a wide range of research applications.Transforming Growth Factor Beta 2 (TGFb2) is a protein that plays a key role in regulating cell growth, proliferation, and differentiation. It is involved in various cellular processes, including immune response, wound healing, and tissue homeostasis.
Dysregulation of TGFb2 has been linked to several diseases, such as fibrosis, cancer, and autoimmune disorders, highlighting its importance as a potential biomarker and therapeutic target.By using the Human TGFb2 ELISA Kit, researchers can gain valuable insights into the role of TGFb2 in health and disease, ultimately advancing our understanding of these complex conditions and paving the way for novel treatment strategies.
Product Name:
Human Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
