Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA Kit
The Human Transmembrane Protease Serine 2 (TMPRSS2) ELISA Kit is a cutting-edge tool for the accurate quantification of TMPRSS2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TMPRSS2 is a key transmembrane serine protease that plays a critical role in viral entry, specifically in facilitating the entry of coronaviruses like SARS-CoV-2 into host cells.
Understanding the expression levels of TMPRSS2 is crucial for studying viral pathogenesis and developing effective antiviral strategies.With its advanced technology and superior performance, the Human TMPRSS2 ELISA Kit is an invaluable tool for researchers studying viral infections, respiratory diseases, and therapeutic interventions targeting TMPRSS2. Unlock new insights into viral pathogenesis and host-virus interactions with this innovative ELISA kit.
Product Name:
Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA Kit
Product Code:
HUDL02925
Size:
96 Assays
Target:
TMPRSS2
Synonyms:
PRSS10, Serine protease 10
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
