Human TRH (Thyrotropin Releasing Hormone) ELISA Kit
The Human TRH (Thyrotropin Releasing Hormone) ELISA Kit is a powerful tool for accurately measuring TRH levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reliable results, making it an invaluable asset for a variety of research applications. TRH, also known as thyrotropin-releasing hormone, is a key regulator of the hypothalamic-pituitary-thyroid axis, playing a crucial role in the release of thyroid-stimulating hormone (TSH) and subsequent thyroid hormone production.
Dysregulation of TRH levels has been linked to thyroid disorders, neuroendocrine abnormalities, and other conditions, underscoring its importance as a biomarker for understanding and treating various diseases. By utilizing the Human TRH ELISA Kit, researchers can gain valuable insights into the role of TRH in physiological processes and diseases, paving the way for the development of targeted therapies and diagnostic tools. Trust in the accuracy and reliability of this kit to advance your research and uncover new possibilities in the field of endocrinology.
Product Name:
Human TRH (Thyrotropin Releasing Hormone) ELISA Kit
SKU:
AEFI00113
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.46 pg/mL
Range:
0.78-50 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-101%
92-97%
89-102%
EDTA Plasma (n = 5)
92-97%
90-101%
83-100%
Heparin Plasma (n = 5)
83-96%
83-99%
84-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
89-101
93
EDTA Plasma (n = 5)
87-100
95
Heparin Plasma (n = 5)
90-104
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
