Description
Human TRIM72 Recombinant Protein (N-His)
Human TRIM72 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu288-Ala477, with an N-terminal His tag. Predicted molecular weight: 23.27 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Tripartite motif-containing protein 72 is a ~52 kDa protein. Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. For research use only.
Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process.
| Product Name: | Human TRIM72 Recombinant Protein (N-His) |
| SKU: | GXRP11643 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:2.3.2.27, MG53, Mg53, Mitsugumin-53, TRIM72, Tripartite motif-containing protein 72 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu288-Ala477 |
| Calculated MW: | 23.27 kDa |
| UniProt: | Q6ZMU5 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
