Tryptase is an enzyme primarily produced by mast cells, and its measurement is essential for diagnosing and monitoring mast cell activation-related disorders, such as anaphylaxis and mastocytosis. The ELISA (Enzyme-Linked Immunosorbent Assay) method allows for the accurate detection and quantification of tryptase by utilizing specific antibodies that bind to the enzyme. The kit provides a reliable and quantitative assessment of tryptase levels, aiding clinicians and researchers in understanding the underlying mechanisms of mast cell-related conditions and facilitating the development of appropriate treatment strategies.
Description
Human Tryptase ELISA Kit
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human Tryptase ELISA Kit |
| Product Code: | HUFI02921 |
| Size: | 96 Assays |
| Alias: | TPS |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human TPS concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.094ng/ml |
| Range: | 0.156-10ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Human TPS and the recovery rates were calculated by comparing the measured value to the expected amount of Human TPS in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of [ANALYTE] and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| CV(%) | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - Related Products
Human Tryptase (TPS) ELISA Kit (HUDL02979)
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Human Tryptase gamma (TPSG1) ELISA Kit (HUEB1026)
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Human Tryptase delta (TPSD1) ELISA Kit (HUEB0478)
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Human Tryptase gamma/TPSG1 ELISA Kit (HUFI00928)
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