The Human TTR (Transthyretin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of transthyretin levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Transthyretin is a vital protein involved in transporting thyroid hormones and vitamin A in the body. Abnormal levels of transthyretin have been linked to various conditions such as amyloidosis, familial amyloidotic polyneuropathy, and Alzheimer's disease.
Therefore, the Human TTR CLIA Kit serves as an essential tool for studying these conditions and developing potential therapies.Overall, the Human TTR CLIA Kit offers researchers a reliable and accurate method for quantifying transthyretin levels, allowing for a deeper understanding of its role in different diseases and potential therapeutic interventions.
Product Name:
Human TTR (Transthyretin) CLIA Kit
SKU:
HUES01212
Target:
Human TTR (Transthyretin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TTR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TTR. You can calculate the concentration of Human TTR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
95-106
100-116
Average (%)
94
101
106
1:4
Range (%)
101-113
96-110
93-108
Average (%)
107
102
99
1:8
Range (%)
93-107
104-120
95-109
Average (%)
98
110
101
1:16
Range (%)
90-104
93-108
89-101
Average (%)
97
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.42
288.84
989.2
110.44
271.01
971.79
Standard deviation
12.37
28.16
78.34
12.41
27.72
77.94
C V (%)
11.62
9.75
7.92
11.24
10.23
8.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TTR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TTR in samples. No significant cross-reactivity or interference between Human TTR and analogues was observed.
