Human Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit
The Human Tumor Necrosis Factor Alpha Induced Protein 6 (TNFAIP6) ELISA Kit is specifically designed for the precise detection of TNFAIP6 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity to ensure accurate and consistent results, making it well-suited for various research purposes.TNFAIP6, also known as TNF-stimulated gene 6 protein, is a key player in inflammatory responses and tissue remodeling. It is involved in various pathologies such as inflammatory diseases, cancer, and tissue injury.
Monitoring TNFAIP6 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With its reliable performance and ease of use, the Human TNFAIP6 ELISA Kit is a valuable tool for researchers studying the role of TNFAIP6 in disease pathogenesis and treatment strategies. Order your kit today to advance your research efforts in the field of inflammatory and oncologic diseases.
Product Name:
Human Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
