Description
Human TWNK Recombinant Protein (N-His)
Human TWNK Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Val384-Lys635, with an N-terminal His tag. Predicted molecular weight: 31.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Twinkle mtDNA helicase (TWNK) is a ~77 kDa protein. Mitochondrial helicase involved in mtDNA replication and repair. For research use only.
Might have a role in mtDNA repair. Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB. Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate. Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand.
| Product Name: | Human TWNK Recombinant Protein (N-His) |
| SKU: | GXRP03255 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | PEO1, Twinkle mtDNA helicase, Progressive external ophthalmoplegia 1 protein, T7-like mitochondrial DNA helicase, TWNK, T7 gp4-like protein with intramitochondrial nucleoid localization, C10orf2, Twinkle protein, mitochondrial |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Val384-Lys635 |
| Calculated MW: | 31.03 kDa |
| UniProt: | Q96RR1 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
